jak2 stat3 pathway inhibitor ag 490  (MedChemExpress)

Journal: Heliyon

Article Title: Jianpi Qingre Tongluo Decoction exerted an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis

doi: 10.1016/j.heliyon.2024.e34634

Figure Lengend Snippet: Network pharmacology and molecular docking of the main components of JQP with the STAT3 protein molecule. A. Venn diagram of candidate target genes of JQP and AS. B. Protein-protein interaction network, the darker the color, the higher the importance. C. Degree screening of the top 20 gene targets. D. Cytoscape 3.7.1 The software draws the active ingredient-target gene network of TCM, in which the yellow square represents the target gene, the green circle represents the traditional Chinese medicine, and the green square represents the active ingredient of the traditional Chinese medicine. E. Bubble chart of KEGG pathway enrichment analysis of candidate AS targets. The size of the dots indicates the number of selected genes, and the color indicates the p value of the enrichment analysis. F. Visualization of molecular docking of active ingredients and core protein targets in the core formulation. (a) Docking diagram of quercetin (MOL000098) and STAT3. (b) Docking diagram of kaempferol (MOL000422) and STAT3. (c) Docking diagram of wogonin (MOL000173) and STAT3. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The JAK2/STAT3 pathway inhibitor AG-490 (10 μm) (MCE, No.: HY-12000) was applied to AS-FLSs for a duration of 24 h. To overexpress NONHSAT227927.1, the NONHSAT227927.1 gene was introduced into the pcDNA3.1 plasmid (GenePharma, Shanghai, China).

Techniques: Software, Formulation

Journal: Heliyon

Article Title: Jianpi Qingre Tongluo Decoction exerted an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis

doi: 10.1016/j.heliyon.2024.e34634

Figure Lengend Snippet: Effects of NONHSAT227927.1 on cell viability, inflammatory cytokines and inflammatory pathways.A, Screening of NONHSAT227927.1 small interfering RNA model. B, CCK-8 method was used to detect the cell viability of AS-FLS. C, RT-qPCR was used to detect the expression of NONHSAT227927.1 in AS-FLS. D-G, ELISA was used to detect the levels of IL-6, IL-17, TNF-α, and IL-10 in AS-FLS. H–I, RT-qPCR was used to detect the levels of JAK2 and STAT3 in AS-FLS. J, WB was used to detect Levels of JAK2, STAT, p -JAK2 and p-STAT3 in AS-FLS. K, IF was used to detect the expression of JAK2 and STAT3 in AS-FLS. All experiments were repeated three times.a: ov-NC, b: ov-NONHSAT227927.1, c: si-NC, d: si-NONHSAT227927.1. ***P < 0.001.

Article Snippet: The JAK2/STAT3 pathway inhibitor AG-490 (10 μm) (MCE, No.: HY-12000) was applied to AS-FLSs for a duration of 24 h. To overexpress NONHSAT227927.1, the NONHSAT227927.1 gene was introduced into the pcDNA3.1 plasmid (GenePharma, Shanghai, China).

Techniques: Small Interfering RNA, CCK-8 Assay, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Jianpi Qingre Tongluo Decoction exerted an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis

doi: 10.1016/j.heliyon.2024.e34634

Figure Lengend Snippet: NONHSAT227927.1/JAK2/STAT3 combination regulates the activity of AS-FLS and the release of inflammatory factors. A, CCK-8 method was used to detect the cell viability of AS-FLSs. B-E, ELISA method was used to detect the levels of IL-6, IL-17, TNF-α, and IL-10 in AS-FLS. FG, RT-qPCR was used to detect Levels of JAK2 and STAT3 in AS-FLS. H, WB was used to detect the levels of JAK2, STAT, p -JAK2 and p-STAT3 in AS-FLS. I, IF was used to detect the expression of JAK2 and STAT3 in AS-FLS. All experiments were repeated three times. a: FLS, b: AG490, c: ov-NC, d: ov-NONHSAT227927.1, e: AG490 + ov-NONHSAT227927.1.*P < 0.05,**P < 0.01,***P < 0.001.

Article Snippet: The JAK2/STAT3 pathway inhibitor AG-490 (10 μm) (MCE, No.: HY-12000) was applied to AS-FLSs for a duration of 24 h. To overexpress NONHSAT227927.1, the NONHSAT227927.1 gene was introduced into the pcDNA3.1 plasmid (GenePharma, Shanghai, China).

Techniques: Activity Assay, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing

Journal: Heliyon

Article Title: Jianpi Qingre Tongluo Decoction exerted an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis

doi: 10.1016/j.heliyon.2024.e34634

Figure Lengend Snippet: JQP reversed the effects of NONHSAT227927.1 overexpression on AS-FLS viability, inflammatory factors and JAK2/STAT3 pathway. A, CCK-8 method was used to screen the optimal concentration of JQP-containing serum. B. Use CCK-8 method to detect the cell viability of AS-FLSs. C. RT-qPCR detects the expression of NONHSAT227927.1. D-G used ELISA to detect the levels of IL-6, IL-17, TNF-α, and IL-10. HI, RT-qPCR was used to detect the expression of JAK2 and STAT3. J. WB detects the expression of JAK2, STAT3, p -JAK2 and p-STAT3 proteins. K. Immunofluorescence method detects the expression of p -JAK2 and p-STAT3 proteins. a: FLS, b: NS, c: JQP, d: ov-NC, e: ov-NONHSAT227927.1, f: ov-NONHSAT227927.1 + JQP. The data is displayed in the form of the average value plus or minus the standard deviation. The experiments were conducted thrice.*P < 0.05,**P < 0.01,***P < 0.001.

Article Snippet: The JAK2/STAT3 pathway inhibitor AG-490 (10 μm) (MCE, No.: HY-12000) was applied to AS-FLSs for a duration of 24 h. To overexpress NONHSAT227927.1, the NONHSAT227927.1 gene was introduced into the pcDNA3.1 plasmid (GenePharma, Shanghai, China).

Techniques: Over Expression, CCK-8 Assay, Concentration Assay, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Standard Deviation

Journal: Heliyon

Article Title: Jianpi Qingre Tongluo Decoction exerted an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis

doi: 10.1016/j.heliyon.2024.e34634

Figure Lengend Snippet: JQP exerts an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis.

Article Snippet: The JAK2/STAT3 pathway inhibitor AG-490 (10 μm) (MCE, No.: HY-12000) was applied to AS-FLSs for a duration of 24 h. To overexpress NONHSAT227927.1, the NONHSAT227927.1 gene was introduced into the pcDNA3.1 plasmid (GenePharma, Shanghai, China).

Techniques:

Journal: BMC Cancer

Article Title: CCL18-NIR1 promotes oral cancer cell growth and metastasis by activating the JAK2/STAT3 signaling pathway

doi: 10.1186/s12885-020-07073-z

Figure Lengend Snippet: AG490 inhibited the CCL18-induced OSCC proliferation, migration, invasion, and EMT. a Western blotting showed that AG490 could effectively inhibit the expression of phosphorylation of both JAK2 and STAT3 in HSC6 cells. b, c and d Results of CCK8 and transwell demonstrated AG490 impaired the proliferation, migration, and invasion of rCCL18-stimulated OSCC cells. e The increased expression of E-cadherin and the decreased expression of N-cadherin and ZEB2 were found in the rCCL18 + AG490 group when compared with the rCCL18 + NC control group. ( *P < 0.05, **P < 0.01, ***P < 0.001, vs. control). The full-length blots are presented in Supplementary Fig. S

Article Snippet: The JAK2/STAT3 signaling pathway specific inhibitor AG490 was purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Migration, Western Blot, Expressing, Phospho-proteomics, Control